Covalent Immobilizations of Myosin for In-Vitro Motility of Actin

Ellis, Bagga and Kumari, Sunita and Kumar, Rajesh and Kumar, Rakesh and Bajpai, R.P. and Bharadwaj, L.M. (2005) Covalent Immobilizations of Myosin for In-Vitro Motility of Actin. PRAMANA-Journal of Physica, 65 (5). pp. 967-972. ISSN 0304-4289

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Abstract

The present study reports the covalent immobilization of myosin on glass surface and in-vitro motility of actin-myosin biomolecular motor. Myosin was immobilized on poly-L-lysine coated glass using heterobifunctional cross linker EDC and characterized by AFM. The in-vitro motility of actin was carried out on the immobilized myosin. It was observed that velocity of actin over myosin increases with increasing actin concentration (0.4{1.0 mg/ml) and was found in the range of 0.40{3.25 ¹m/s. The motility of actin- myosin motor on arti¯cial surfaces is of immense importance for developing nanodevices for healthcare and engineering applications.

Item Type:	Article
Uncontrolled Keywords:	Molecular motors; atomic force microscope(AFM); immobilization; biomolecular electronics and nanotechnology.
Subjects:	CSIO > Nano Science and Nano Technology
Depositing User:	Ms. J Shrivastav
Date Deposited:	03 Apr 2012 10:09
Last Modified:	10 Apr 2012 16:25
URI:	http://csioir.csio.res.in/id/eprint/13

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