Catechol Biosensor Based on Gold Nanoparticle Modified Tetrabutyl ammonium tetrafluoroborate Doped Polythiophene

Singh, Suman and Kumar, Parveen S. and Jain, D.V.S. and Singla, M.L. (2010) Catechol Biosensor Based on Gold Nanoparticle Modified Tetrabutyl ammonium tetrafluoroborate Doped Polythiophene. Sensors & Transducers Journal, 122 (11). pp. 85-101. ISSN 1726-5479

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Abstract

Tetra butyl ammonium tetra fluoroborate (TBATFB) doped polythiophene films have been polymerized galvano-statically which were then modified with soium citrate capped gold nanoparticles (AuNPs) using dip coating method. Catechol biosensor was fabricated using laccase enzyme. ITO/Pth and ITO/Pth/AuNPs/Lac bioelectrodes were characterized using scanning electron microscopy (SEM), UV-Visible spectroscopy and electrochemical techniques. Absorbance for ITO/Pth/AuNPs/Lac bioelectrodes was monitored at 410 nm as a function of catechol concentration and pH. Cyclic voltammetric studies of ITO/Pth electrodes showed quasi-reversible behavior when recorded in sodium acetate buffer, which later turned into reversible behavior on modification with gold nanoparticles. ITO/Pth/AuNPs/Lac bioelectrodes showed maximum oxidation potential for enzymatic reaction of catechol at -0.2 V, with linear range upto 0.8 mM and regression coefficient (R2) of 0.988. Sensitivity and Km values were found to be 3.7 x 10-5 mA/mM and 0.22 mM respectively, with response time of about 6-8 s Suman SINGH, aS. Praveen KUMAR, bD. V. S. JAIN, a*M. L. SINGLA

Item Type: Article
Uncontrolled Keywords: Polythiophene, Gold nanoparticles (AuNPs), Catechol, Laccase
Subjects: CSIO > Chemistry
Depositing User: Ms T Kaur
Date Deposited: 02 Feb 2012 14:52
Last Modified: 10 Apr 2012 15:05
URI: http://csioir.csio.res.in/id/eprint/139

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