1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide interference with Lowry method

Kumar, Rakesh and Shukla, A.K. and Bagga, E. and Kumari, S. and Bajpai, R.P. and Bharadwaj, L.M. (2005) 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide interference with Lowry method. Analytical Biochemistry, 336 (1). pp. 132-134. ISSN 0003-2697

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Official URL: http://www.ncbi.nlm.nih.gov/pubmed/15582568

Abstract

1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was tested for interference with the Lowry method of protein determination. The EDC interference was nearly additive at both 660 and 750 nm. Blue-colored complex developed in the case of EDC and showed maximum absorbance at 750 nm, similar to the bovine serum albumin (BSA) estimation. In time response analysis, blue-colored complex was developed after 30min of incubation in both cases (EDC and BSA); therefore, it followed the same kinetic pattern of color development as that of BSA. Interference is believed to be caused by the reduction of the folin–ciocalteu reagent by EDC, resulting in increased blue-colored complex and apparently causing increased protein content of the sample.

Item Type: Article
Uncontrolled Keywords: 1-Ethyl-3-(3-dimethylaminopropyl);Blue-colored complex;bovine serum albumin;cases;color development;EDC;incubation;kinetic pattern;Lowry method;maximum absorbance;time response analysis
Subjects: CSIO > Biochemistry
Depositing User: Ms. Narinder Kaur
Date Deposited: 09 Feb 2012 16:24
Last Modified: 11 Apr 2012 14:27
URI: http://csioir.csio.res.in/id/eprint/14

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