Kaur, Harsimran and Das, Tapan and Kumar, Rajesh and Ajore, Ram and Bharadwaj, L.M. (2008) Covalent attachment of actin filaments to Tween 80 coated polystyrene beads for cargo transportation. Biosystems, 92 (1). pp. 69-75. ISSN ISSN: 0303-2647
Full text not available from this repository.Abstract
In this manuscript, a new strategy has been reported for circumscribed covalent attachment of barbed and pointed ends of actin filaments to polystyrene beads. A comparative study of attachment of actin filaments to polystyrene beads was performed by blocking functionally active sites on polystyrene beads with nonionic detergents such as Tween 20, Tween 80 and polyethylene glycol (PEG). Effective blocking of active sites was obtained with Tween 80 at 0.1% concentration. Attachment of single bundle of actin filament to bead was assessed by rotational motion of bead tailed actin in front and lateral view. Velocity of actin filaments attached to different size of beads in in-vitro motility assay was calculated to ascertain their attachments. Velocity of actin attached to 1.0 and 3.0 microm polystyrene beads was reduced to 3.0-4.0 and 0.0-1.0 microm/s, respectively as compared to free actin velocity of 4.0-6.0 microm/s. Single point attachment of actin filaments to different size of beads was assessed by decrease in sliding velocity. Present study provides insight into the actin-myosin based molecular motor systems for drug delivery and biosensors applications.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Molecular motors; Covalent attachment; Cargo transportation; In vitro motility assay; Drug delivery |
| Subjects: | CSIO > Nano Science and Nano Technology |
| Divisions: | Nano Science and Nano Technology |
| Depositing User: | Ms T Kaur |
| Date Deposited: | 23 Jan 2012 16:49 |
| Last Modified: | 10 Apr 2012 15:22 |
| URI: | http://csioir.csio.res.in/id/eprint/204 |
Actions (login required)
 |
View Item |
