Immobilization of enzyme on long period grating fibers for sensitive glucose detection

Deep, Akash and Tiwari, Umesh and Kumar, Parveen and Mishra, Vandana and Jain, S.C. and Singh, Nahar and Kapur, Pawan and Bharadwaj, L.M. (2012) Immobilization of enzyme on long period grating fibers for sensitive glucose detection. Biosensors and Bioelectronics, 33 (1). pp. 190-195. ISSN 0956-5663

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Glucose oxidase (GOD) immobilized longperiodgrating (LPG) fibers have been proposed for the specific and sensitivedetection of glucose. The treatment of LPG fibers with aminopropyl triethoxysilane has induced biding sites for the subsequent GOD immobilization. Field emission scanning electron microscopy, confocal laser scanning microscopy, infrared spectroscopy and Raman spectroscopy have provided detailed evidences about the effectiveness of the adopted biofunctionalization methodology. The enzyme activity is conserved during the immobilization step. Fabricated LPG sensor was tested on different glucose solutions to record the transmission spectra on an optical spectrum analyzer. The wavelength shifts in the transmission spectra are linearly correlated with the glucose concentration in the range of 10–300 mg dL−1. The fabricated sensor gives fast response and is demonstrated to be of practical utility by determining glucose contents in blood samples. Proposed technique can further be extended to develop LPG fiber based novel, sensitive and label free nanosensors for disease diagnosis and clinical analysis.

Item Type: Article
Uncontrolled Keywords: Longperiodgrating;Glucose oxidase;FTIR spectroscopy; Raman spectroscopy; Biosensing;Glucose
Subjects: CSIO > Applied Physics
Depositing User: Ms. J Shrivastav
Date Deposited: 23 Apr 2012 11:12
Last Modified: 23 Apr 2012 11:12

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