Transportation of drug-gold nanocomposites by actinomyosin motor system

Kaur, Harsimran and Chaudhary, Archana and Kaur, Inderpreet and Singh, Kashmir and Bharadwaj, L.M. (2011) Transportation of drug-gold nanocomposites by actinomyosin motor system. Journal of Nanoparticle Research, 13 (6). pp. 2295-2303. ISSN 1388-0764 (print) 1572-896X (online)

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Nanotechnology is playing an important role in drug delivery to overcome limitations of conventional drug delivery systems in terms of solubility, in vivo stability, pharmacokinetics, and biodistribution. The controlled transportation of drug into the cell and within the cell is a major challenge to be addressed. Cellular molecular motors have been exploited for their cargo carrying capacity for various applications including engineering and health care. Combination of nanotechnology and biomolecular motors can address some of the challenges in drug delivery. In the present study, transportation of drug nanocomposites has been demonstrated. Nanocomposites of 6-mercaptopurine and levodopa drugs (cancer and Parkinson’s disease, respectively) were prepared with gold nanoparticles (GNPs) by covalent attachment and these nanocomposites were attached to actin filaments. These nanocomposites were in-turn transported by actin filaments on myosin tracks. Characterization of drug nanocomposites formation was done by UV–Vis spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and confocal microscopy. GNP composites of 6-mercaptopurine and levodopa were formed by sulfide and amide bond formation, respectively. Average velocity of actin filament attached to nanocomposites was found to be 3.17 and 3.89 lm/s for levodopa and 6-mercaptopurine, respectively, as compared to actin filaments with velocity of 4.0–6.0 lm/s. Three concepts have been proposed for the study of drug transportation into the cell based on polycationic complex formation, interaction of actin with cellular myosin and Biomolecular Adaptor for Retrograde Transport (BART) technology. The aspects of this study heads toward the development of an approach to utilize molecular motors for nanoscale transportation endogenously.

Item Type: Article
Uncontrolled Keywords: Actin Myosin; Gold nanoparticle;Levodopa;6-Mercactopurine; Nanocomposites; Nanobiotechnology; Nanomedicine
Subjects: CSIO > Nano Science and Nano Technology
Depositing User: Ms. J Shrivastav
Date Deposited: 21 Apr 2012 23:16
Last Modified: 21 Apr 2012 23:16

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