Nanostructured polyaniline–silicon substrate for protein biosensing

Deep, Akash and Sharma , A.L. and Kumar, Parveen and Bharadwaj, L.M. (2012) Nanostructured polyaniline–silicon substrate for protein biosensing. Sensors and Actuators B: Chemical, 171-72. pp. 210-215. ISSN 0925-4005

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Polyaniline (PAni) has been electrochemically deposited on a silicon (Si) substrate to form thin nanostructured films of around 50 nm thickness. These films have been modified with avidin, followed by the immobilization of biotinylated anti-human IgG to form a sensing bioelectrode. The deposited PAni thin films on the Si substrate have been characterized by electron and atomic force microscopy. Successful immobilization of the biomolecules has been confirmed by Raman spectrometric studies. The current–voltage characteristics of the prepared sensor are exploited for the determination of human IgG antigen in the concentration range of 5–550 μg mL−1. The proposed biosensor's detection limit and sensitivity are 5 μg mL−1 and 0.15 μS ppm−1 human IgG, respectively.

Item Type: Article
Uncontrolled Keywords: Polyaniline; Electrochemical; Biofunctionalization; Thin films; Anti-human IgG; Immunosensing
Subjects: CSIO > Nano Science and Nano Technology
Divisions: Nano Science and Nano Technology
Depositing User: Ms. J Shrivastav
Date Deposited: 01 Feb 2013 03:58
Last Modified: 01 Feb 2013 03:58

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