Rapid HPLC method development for determination of vitamin C, phenolic acids, hydroxycinnamic acids, and flavonoids in Emblica officinalis juice

Bansal, Vasudha and Sharma, Anupma and Ghanshyam, C. and Singla, M.L. (2014) Rapid HPLC method development for determination of vitamin C, phenolic acids, hydroxycinnamic acids, and flavonoids in Emblica officinalis juice. Journal of Liquid Chromatography & Related Technologies, 38 (5). pp. 619-624. ISSN 1082-6076 (Print), 1520-572X (Online)

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Official URL: http://www.tandfonline.com/doi/abs/10.1080/1082607...

Abstract

The work proposes a simple method for simultaneous detection and quantification of vitamin C (ascorbic acid), phenolic acids (gallic acid and ellagic acid), hydroxycinnamic acid (chlorogenic acid), and flavonoids (myricetin, quercetin, and kaempferol) in seasonal samples of emblica juice. The compounds were separated by an intangible curved gradient of 0.1% orthophosphoric acid in water (v/v) and acetonitrile as mobile phase A and B using Zorbax SB RP C-18 column at a wavelength of 254 nm in 18 min. The assay was optimized by varying the mobile phase, gradient type, and detection wavelength. The method was validated in terms of linearity, precision, detection limits, and quantification limits. Good linear response was observed over the range specified for all the analytes, as confirmed by the correlation coefficient which ranged from 0.991 and 0.995. The limit of detection and limit of quantification were found to be in the range of 0.129–0.685 µg mL−1 and 0.43–2.883 µg mL−1, respectively. Complete resolution of the phenolic compounds was achieved without the need of pre-analysis processes. The overall precision values obtained for standards and samples were within the range of 0.01–0.2 and 0.15–0.23, respectively.

Item Type: Article
Uncontrolled Keywords: curved gradient, Emblica officinalis juice, HPLC, method development, total polyphenol content, vitamin C
Subjects: CSIO > Agri - Instrumentation
Divisions: Agri - Instrumentation
Depositing User: Ms. Jyotsana
Date Deposited: 09 Aug 2018 12:09
Last Modified: 09 Aug 2018 12:09
URI: http://csioir.csio.res.in/id/eprint/581

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