Quantification of Human Immunoglobulin G Immobilised of Gold Coated Silicon Chip for Biosensing Applications

Vashist, S.K. and Raiteri, R. and Tiwari, R. and Bajpai, R.P. and Bharadwaj, L.M. (2006) Quantification of Human Immunoglobulin G Immobilised of Gold Coated Silicon Chip for Biosensing Applications. Journal of Physics: Conference Series, 34. pp. 806-811. ISSN 17426588

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Abstract

The most important aspect of biosensor development with high sensitivity is the oriented immobilization of antibodies on the solid substrate. Human IgG was immobilized on gold-coated silicon employing protein A, protein G and neutravidin immobilization procedures. The amount of human IgG immobilized was analyzed by 3, 3ƍ, 4, 4ƍ- tetramethyl benzidine (TMB) substrate assay and was maximum when protein A immobilization procedure was followed. Human IgG coated biosensing surface was regenerated by treatment with glycine-HCl buffer (50 mM, pH 2.2). Atomic force microscopy was used to analyze the distribution of biomolecules immobilized on gold-coated silicon. Our study indicates that human IgG molecules were uniformly bound to gold-coated silicon by protein A immobilization procedure

Item Type:	Article
Subjects:	CSIO > Nano Science and Nano Technology
Depositing User:	Ms. J Shrivastav
Date Deposited:	03 Apr 2012 10:11
Last Modified:	10 Apr 2012 16:24
URI:	http://csioir.csio.res.in/id/eprint/62

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